27 August 2015

#97 Summary of Energy and Respiration

1 Organisms must do work to stay alive. The energy input necessary for this work is either light, for photosynthesis, or the chemical potential energy of organic molecules. Work includes anabolic reactions, active transport and movement. Some organisms, such as mammals and birds, use thermal energy released from metabolic reactions to maintain their body temperature. 

 2 Reactions that release energy must be harnessed to energy-requiring reactions. Th is ‘harnessing’ involves an intermediary molecule, ATP. Th is can be synthesised from ADP and phosphate using energy, and hydrolysed to ADP and phosphate to release energy. ATP therefore acts as an energy currency in all living organisms. 

 3 Respiration is the sequence of enzyme-controlled steps by which an organic molecule, usually glucose, is broken down so that its chemical potential energy can be used to make the energy currency, ATP. 

 4 In aerobic respiration, the sequence involves four main stages: glycolysis, the link reaction, the Krebs cycle and oxidative phosphorylation. 

 5 In glycolysis, glucose is fi rst phosphorylated and then split into two triose phosphate molecules. Th ese are further oxidised to pyruvate, giving a small yield of ATP and reduced NAD. Glycolysis occurs in the cell cytoplasm. 

 6 When oxygen is available (aerobic respiration), the pyruvate passes to the matrix of a mitochondrion. There, in the link reaction, pyruvate is decarboxylated and dehydrogenated and the remaining 2C acetyl unit combined with coenzyme A to give acetyl coenzyme A. 

 7 The acetyl coenzyme A enters the Krebs cycle in the mitochondrial matrix and donates the acetyl unit to oxaloacetate (4C) to make citrate (6C). 

 8 The Krebs cycle decarboxylates and dehydrogenates citrate to oxaloacetate in a series of small steps. Th e oxaloacetate can then react with another acetyl coenzyme A from the link reaction.

9 Dehydrogenation provides hydrogen atoms, which are accepted by the carriers NAD and FAD. Th ese pass to the inner membrane of the mitochondrial envelope, where they are split into protons and electrons. 

 10 In the process of oxidative phosphorylation, the electrons are passed along a series of carriers. Some of the energy released in this process is used to move protons from the mitochondrial matrix to the intermembrane space. This sets up a gradient of protons across the inner membrane of the mitochondrial envelope. The protons pass back into the matrix, moving down their concentration gradient through protein channels in the inner membrane. An enzyme, ATP synthase, is associated with each of these channels. ATP synthase uses the electrical potential energy of the proton gradient to phosphorylate ADP to ATP.

11 At the end of the carrier chain, electrons and protons are recombined and reduce oxygen to water.

12 In the absence of oxygen as a hydrogen acceptor (in anaerobic respiration), a small yield of ATP is made by dumping hydrogen into other pathways in the cytoplasm which produce ethanol or lactate. The lactate pathway can be reversed in mammals when oxygen becomes available. The oxygen needed to remove the lactate produced during anaerobic respiration is called the oxygen debt. 

 13 The energy values of respiratory substrates depend on the number of hydrogen atoms per molecule. Lipids have a higher energy density than carbohydrates or proteins. 

14 The respiratory quotient (RQ) is the ratio of the volumes of oxygen absorbed and carbon dioxide given off in respiration. The RQ reveals the nature of the substrate being respired. Carbohydrate has an RQ of 1.0, lipid 0.7 and protein 0.9. 

 15 Oxygen uptake, and hence RQ, can be measured using a respirometer. 

1. End-of-chapter questions

1. What does not occur in the conversion  of glucose to two molecules of pyruvate?

A hydrolysis of ATP
B phosphorylation of ATP
C  phosphorylation  of triose (3C) sugar
D reduction of NAD

 2   Wheredoes each stage of aerobic respiration  occur in a eukaryotic  cell?

3   The  diagram  summarises  anaerobic  respiration.

4    Distinguish    between:

a    an  energy   currency    molecule   and  an energy   storage   molecule.
b    decarboxylation    and  dehydrogenation.

5    State  the  roles  in  respiration    of:

a    NAD
b    coenzymeA
c    oxygen.

6    Copy  and  complete    the  table  to  show  how  much   ATP  is used  and  produced    for  each  molecule   of glucose  respired in the  various   stages  of respiration.

7   a    Explain  why  the  energy  value  of lipid  is more  than   twice  that  of carbohydrate.[2]

b   Explain  what  is meant   by respiratory    quotient    (RQ).                                           [2]
c    Copy and  complete  the  table  to show  that  different respiratory substrates have different   RQs.


d   Measurements     of oxygen  uptake   and  carbon   dioxide   production     by germinating     seeds  in  a respirometer    showed that  25 cm3 of oxygen  was used  and 17.5 cm3 of carbon   dioxide   was  produced    over  the  same  time  period.

   i Calculate   the  RQ  for  these  seeds.   [2]                                                                                                                                      
  ii Identify the  respiratory    substrate   used  by the  seeds.      [1]                                                                                                            
e    Dahlia   plants   store  a compound     called  inulin,   which   is a polymer   of fructose.   The  structure    of fructose   is shown  in the  diagram.

Calculate   the  RQ  when   inulin   is hydrolysed    and  then  respired   aerobically.                   [2]
[Total:   12]

2. End-of-chapter answers

1 C

2 D

3 D

4 a energy currency: immediate donor of energy to all energy-requiring reactions in a cell;
    energy storage: short-term (glucose, sucrose) or long-term (starch, glycogen, triglyceride) store of                                  chemical potential energy;
b decarboxylation: a reaction in which carbon dioxide is removed from a compound;
     dehydrogenation: a reaction in which hydrogen is removed from a compound;

5 a NAD: a hydrogen carrier molecule: it accepts a hydrogen from one reaction and donates it to another;

 b coenzyme A: a carrier of an acetyl group from the link reaction to the Krebs cycle;

 c oxygen: the final electron acceptor and hydrogen ion acceptor in oxidative phosphorylation: the oxygen is reduced to water;

Exam-style questions

 7 a Lipid has more hydrogen atoms per molecule than does carbohydrate;
      most energy liberated in aerobic respiration comes from the oxidation of hydrogen to water; [2]

8 a provide hydrogen to reduce NAD and FAD;
    reduced carriers pass to electron transport chain;
     provide energy for ATP synthesis in oxidative phosphorylation;
     refer to chemiosmosis;                                          [max. 3]

 b i increasing the concentration of aluminium ions from 0 to 40 μmol increases rate of
      fumarate production;
    increases from 40 to 120 μmol have little eff ect; [2]
    ii aluminium binds to enzyme/refer to cofactor; optimises shape of active site; [2]
   [Total: 7]


  1. thank you your notes are actually very helpful. God bless!

  2. thanks!The notes are very helpful but i think the use of diagramatic explanations is very helpful . GOD bless.

  3. thank you very much for the notes, you helped me

  4. Notes are very helpful.... My doubts are clear from these notes and they are easily understandable.

  5. So much helpful qtns n answers m grateful

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