#111 Production of urine in a nephron - Ultrafiltration and reabsorption

Ultrafiltration occurs at the barrier between the blood and the filtrate in the renal capsule or Bowman's capsule in the kidneys.












Ultrafiltration

The Bowman's capsule contains a dense capillary network called the glomerulus. Blood flows into these capillaries through the afferent arteriole and leaves through the efferent arteriole.

The blood in a glomerulus is separated from the space inside the renal capsule by:
• the capillary wall (endothelium) which is one cell thick and has pores in it;
• the basement membrane of the wall of the renal capsule;
• the layer of cells making up the wall of the renal capsule, called podocytes; these cells have slits between them.

The blood in a glomerulus is at a relatively high pressure, because the efferent arteriole is narrower than the afferent arteriole. This forces molecules from the blood through these three structures, into the renal capsule. 

The pores in the capsulary endothelium and the slits between the podocytes will let all molecules through, but the basement membrane acts as a filter and will only let small molecules pass through.

- Substances that can pass through include water, glucose, inorganic ions such as Na+, K+ and Cl- and urea.

- Substances that cannot pass through include red and white blood cells and plasma proteins (such as albumen and fibrinogen).

- The liquid that seeps through into the renal capsule is called glomerular filtrate.

Selective reabsorption in the proximal convoluted tubule

Some of the substances that are filtered into the renal capsule need to be retained by the body. These include:

• much of the water
• all of the glucose
• some of the inorganic ions

These substances are therefore taken back into the blood through the walls of the proximal convoluted tubule. This is called selective reabsorption.

The cells in the walls of the tubule have many mitochondria, to provide ATP for active transport. Their surfaces facing the lumen of the tubule have a large surface area provided by microvilli.

• Active transport is used to move Na+ out of the outer surface of a cell in the wall of the proximal convoluted tubule, into the blood.
• This lowers the concentration of Na+ inside the cell, so that Na+ ions diffuse into the cell from the fluid inside the tubule. The Na+ ions diffuse through protein transporters in the cell surface membrane of the cell
• As the Na+ions diffuse through these transporter proteins, they carry glucose molecules with them. This is called co-transport. The glucose molecules move through the cell and diffuse into the blood.
• The movement of Na+ and glucose into the blood decreases the water potential in the blood. Water therefore moves by osmosis from the fluid inside the tubule, down a water potential gradient through the cells making up the wall of the tubule and into the blood.

Aa a result, the fluid inside the nephron now has:

• no glucose
• a lower concentration of Na+than the filtrate originally had
• less water than the filtrate originally had

About 50% of the urea is also reabsorbed in the proximal convoluted tubule.

The loop of Henle

Some, but not all, nephrons have long loops of Henle that dip down into the medulla and then back up into the cortex. The function of the loop of Henle is to build up a high concentration of Na+ and CI- in the tissues of the medulla. This allows highly concentrated urine to be produced. Note that the loop of Henle itself does not produce highly concentrated urine.

As fluid flows down the descending limb of the loop of Henle, water moves out of it by osmosis. By the time the fluid reaches the bottom of the loop, it has a much lower water potential than at the top of the loop. As it flows up the ascending limb, Na+ and Cl- move out of the fluid into the surrounding tissues, first by diffusion and then by active transport.

This creates a low water potential in the tissues of the medulla. The longer the loop, the lower the water potential that can be produced.

Role of loop of Henle 

• Creating a Salt Gradient in the Medulla
• The function of the loop of Henle is to create a salt bath concentration in the fluid surrounding the tubule
• The descending limb of the loop of Henle is permeable to water, but relatively impermeable to Na+Cl-.
• The ascending limb of the loop of Henle is permeable to salts, but impermeable to water
• This means that as the loop descends into the medulla, the interstitial fluid becomes more salty (and less salty as it ascends into the cortex)
• As the vasa recta blood network that surrounds the loop flows in the opposite direction (counter-current exchange), this further multiplies the effect.

The distal convoluted tubule and collecting duct

The fluid inside the tubule as it leaves the loop of Henle and moves into the collecting duct has lost a little more water and more Na+ than it had when it entered the loop. Because more water has been lost, the concentration of urea has increased.

Now, in the distal convoluted tubule, Na+ is actively transported out of the fluid.

The fluid then flows through the collecting duct. This passes through the medulla, where you have seen that a low water potential has been produced by the loop of Henle. As the fluid continues to flow through the collecting duct, water moves down the water potential gradient from the collecting duct and into the tissues of the medulla. This further increases the concentration of urea in the tubule. The fluid that finally leaves the collecting duct and flows into the ureter is urine.

VIDEO

Homeostasis in humans 

Syllabus 2016-2018 14.1  Homeostasis in mammals Homeostasis in mammals requires complex systems to maintain  internal conditions near  constant. The kidneys  remove wastes from the blood and are the effectors for controlling the water potential of the blood. a)   discuss the importance of homeostasis in mammals and explain the principles  of homeostasis in terms of internal and external stimuli, receptors, central  control,  co-ordination systems, effectors (muscles and glands) b)   define  the term  negative feedback and explain how it is involved in homeostatic mechanisms c)   outline  the roles of the nervous system and endocrine system in co-ordinating homeostatic mechanisms, including thermoregulation, osmoregulation and the control of blood glucose concentration d)   describe the deamination of amino  acids  and outline  the formation of urea  in the urea  cycle (biochemical detail of the urea  cycle is not required) e)   describe the gross structure of the kidney and the detailed structure of the nephron with its associated blood vessels using photomicrographs and electron micrographs f) describe how the processes of ultrafiltration and selective reabsorption are involved with the formation of urine in the nephron g)   describe the roles of the hypothalamus, posterior pituitary, ADH and collecting  ducts in osmoregulation h)   explain how the blood glucose concentration is regulated by negative feedback control mechanisms, with reference to insulin and glucagon i) outline  the role of cyclic AMP as a second messenger with reference to the stimulation of liver cells by adrenaline and glucagon j) describe the three main stages of cell signalling in the control of blood glucose by adrenaline as follows: •   hormone-receptor interaction at the cell surface •   formation of cyclic AMP which binds to kinase  proteins • an enzyme cascade involving activation  of enzymes by phosphorylation to amplify the signal k)   explain the principles  of operation of dip sticks  containing glucose oxidase and peroxidase enzymes, and biosensors that can be used for quantitative measurements of glucose in blood and urine l) explain how urine analysis  is used in diagnosis with reference to glucose, protein  and ketones 14.2  Homeostasis in plants Stomatal aperture is regulated in response to the requirements for uptake of carbon  dioxide for photosynthesis and conserving water. a)   explain that  stomata have daily rhythms of opening and closing and also respond to changes in environmental conditions to allow diffusion of carbon  dioxide and regulate water loss by transpiration b)   describe the structure and function  of guard cells and explain the mechanism by which they  open  and close  stomata c)   describe the role of abscisic acid in the closure of stomata during times of water stress (the role of calcium  ions as a second messenger should  be emphasised)